Table 3.

Applied HA hydrogels scaffolding technologies in Cancer disease.

Chemistry	Models	Scaffold Application	Biological Assessment	Properties Assessment	Biological Assay	Ref.
HA-methacrylate	2D/3D	Brain matrix-mimetic platform	373-MG human GBM cells U87-MG human GBM cells Rat C6 glioma cells	Swelling Elastic modulus Gel microstructure (SEM)	Cell viability	[101]
HA-methacrylate	2D/3D	Inhibition of cell migration	373-MG human GBM cells U87-MG human GBM cells		Time-lapse Microscopy Analysis of Cell Migration Cell Morphological Analyses	[105]
HA-acrylate HA-reactive thiols	2D/3D	ECM-mimetic platform	PCa human prostate adenocarcinoma cells	Swelling Rheological behavior Degradation	Cell Viability Cell proliferation Cell Morphological Analyses Cell-cell adhesion Expression of angiogenic factors	[108]
HA-RGD	3D	ECM-mimetic platform Alkylating chemotherapies drugs treatment	GBM cell lines derived from patients		Cell proliferation Expression of BCL-2 family factors Cell Morphological Analyses	[110]
HA-methacrylate-RGD	2D	3D Matrix stiffness Drug resistance	MDA-MB-231 breast cancer cells	Swelling Elastic Modulus Mesh Size	Cell proliferation Cell Morphological Analyses	[113,114]
HA-methacrylate	2D/3D	ECM-mimetic platform	Patient-derived D456 ​cells U87-MG human GBM cells		Cell proliferation Apoptosis Assay Cell Morphological Analyses Stemness markers expression	[116]