Table 6.
Applied HA hydrogels scaffolding technologies in Drug Delivery.
| Chemistry | Scaffold Application | Biological Assessment | Properties Assessment | Biological Assay | Ref. |
|---|---|---|---|---|---|
| HA-methacrylate HA-β-cyclodextrin MeHA-β-cyclodextrin HA-1-adamantane aceti MeHA-1-adamantane aceti |
Hydrogel protocols for cell and drug encapsulation | MSCs human mesenchyme-mal stromal cells | Fluorescent derivatization Rheological behavior Protein quantification Biomolecule release |
Cell viability | [172] |
| HA-adipic dihydrazide (ADH) | Formulation to attract glioma cells Anticancer drug delivery |
U87-MG human GBM cells | Nuclear magnetic resonance (NMR) Rheological behavior In vitro drug release |
Cell viability Boyden chamber for chemotactic invasion Cloning rings for invasion assay CD44 and MMP9 protein levels |
[174] |
| HA-chitosan/β-glycerophosphate (CS/HA/GP) | Hydrogels for pH sensitive drug release | Hela cancer cells | Rheological behavior Fourier transform infrared (FTIR) Scaffold microstructure (SEM) In vitro drug release Thermal sensitivity and injectability |
CD44 protein levels | [175] |
| S-NSA/A-HA S-NSA/A-HA/cisplatin (CDDP) CS-NSA/A-HA/doxorubicin (DOX) CS-NSA/A-HA/CDDP/DOX |
Hydrogel for the pH-responsive Co-delivery | A549 human lung cancer cells | Drug loading efficiency (DLE) Drug encapsulation efficiency (DEE) In vitro drug release FT-IR spectra Zeta potential values of hydrogel |
Cell viability Cell Morphological Analyses |
[180] |
| HA-bisphosphonate HA-BP-metal-organic framework (MOF) HA-BP·MOF@DOX |
Metal-organic framework (MOF) as a carrier | CT-26 colon carcinoma cells | Nuclear magnetic resonance (NMR) Scaffold microstructure (SEM) |
Cell viability Caspase-3 protein levels In vivo antitumor efficacy by analysis of tumor size Histological analysis |
[188] |
| HA-dopamine (DA) HA-3-(4-hydroxyphenyl) propionic acid (HPA) HA-DA-HPA |
Injectable hydrogel for neural regeneration | Human GFP-MSC cells Human iPSC-derived NSCs |
Fourier transform infrared (FTIR) Nuclear magnetic resonance (NMR) Rheological behavior Scaffold microstructure (SEM) |
Cell viability Pax6, Nestin and βIII-Tubulin gene expression |
[189] |