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. 2022 Aug 2;4(1):vdac120. doi: 10.1093/noajnl/vdac120

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© The Author(s) 2022. Published by Oxford University Press, the Society for Neuro-Oncology and the European Association of Neuro-Oncology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 4. — The interaction of SPRIGHTLY with PTBP1 regulates SMYD3 exon skipping. The group 4 cell line was transfected with siPTBP1 or siNEG or PTBP1 or SPRIGHTLY was overexpressed to determine their effects on exon skipping. (A) The splicing variant lacking exon 5 was detected. Two independent experiments are shown. (B) The expression levels of full-length SMYD3 and SMYD3 E5 skipped (SMYD3 ΔE5) transcripts were determined in tissue RNA-seq data (left and middle panel). The ratio between SMYD3 ΔE5 and full length SMYD3 transcripts in each patient was determined (right panel). siNEG: siRNA for scrambled sequence, siPTBP1: siRNA for PTBP1, V5-PTBP1: V5 epitope-tagged PTBP1. Student’s t-test compared with normal cerebellum (CB). ns > .05, * ≤ .05, **** ≤ .0001.