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. 1999 Mar;6(2):236–242. doi: 10.1128/cdli.6.2.236-242.1999

FIG. 2.

FIG. 2

(A) Evaluation of the purities of different ch14.18-IL-2 fusion protein preparations by SDS-PAGE. Lane 1, molecular size markers (94, 67, 43, 30, and 20 kDa); lane 2, purified ch14.18; lane 3, ch14.18-IL-2 (lot 1); lane 4, ch14.18-IL-2 (lot 31403); lane 5, lower-molecular-weight derivative of the ch14.18-IL-2 fusion protein (an aliquot from a partially purified batch of ch14.18-IL-2 that had been obtained from a cell line which was maintained in the presence of fetal calf serum). (B) Western blot analysis with anti-human IgG1 antibody conjugated with horseradish peroxidase. Lane 1, purified ch14.18; lane 2, ch14.18-IL-2 (lot 1); lane 3, ch14.18-IL-2 (lot 31403); lane 4, lower-molecular-weight derivative of the ch14.18-IL-2 fusion protein.