TABLE 5.
ELISA for: | Concn (ng/ml)
|
% Expected | |
---|---|---|---|
Expected | Observed | ||
1A7-IL-2 | 7,500 | 6,882 | 92 |
IgG | 6,250 | 5,673 | 91 |
IL-2 | 1,250 | 949 | 76 |
Three separate experiments were performed with the ch14.18-IL-2 fusion protein (lot 31403) diluted in a sample buffer and tested with all three ELISA methods. The stock fusion protein concentration was standardized by using the IgG1 ELISA. This was diluted with PBS-Tween-milk so that 7,500 ng of fusion protein per ml was tested in the assay. The expected concentrations of IL-2 and ch14.18 components of the fusion protein were based on the calculation that the IL-2 component is one-sixth and the antibody component is five-sixths of the mass of the fusion protein. Observed data are presented as the means of three experiments. Standard deviations are 508 ng/ml for the 1A7-IL-2 ELISA, 32 ng/ml for the IgG ELISA, and 90 ng/ml for the IL-2 ELISA. Percent expected was calculated as the observed concentration/expected concentration × 100.