Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Aug 11;44(11):1353–1361. doi: 10.1007/s13258-022-01298-x

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 2 — UHRF1 is acetylated by TIP60. a GFP-UHRF1 and FLAG-TIP60 were co-transfected into HCT116 cells. The cell lysates were immunoprecipitated with anti-pan-acetyl antibodies and immunoblotted with indicated antibodies. b TIP60 was stably knocked down in HCT116 cells. HCT116 cell extract of control and shTIP60 were immunoprecipitated with anti-pan-acetyl antibodies and immunoblotted with indicated antibodies. c Full-length GST-fusion UHRF1 was incubated with [¹⁴C]-acetyl-CoA and GST-fusion TIP60 for 3 h at 30 ℃. The samples were separated by SDS-PAGE and stained with Coomassie brilliant blue (CBB) or visualized by autoradiography.