Fig. 3.

TIP60 acetylates UHRF1 4KR residues of SRA-RING domain. a TIP60-mediated acetylated lysine residues within UHRF1 protein were predicted by a web server for KAT-specific Acetylation Site Prediction (ASEB). b Partial recombinant constructs of UHRF1 were used as substrates for in vitro acetylation assays with GST-TIP60. * means acetylated UHRF1. c WT and 4KR of UHRF1 protein were incubated with and [¹⁴C]- acetyl-CoA and recombinant TIP60 for 3 h at 30 ℃. The samples were separated by SDS-PAGE and stained by CBB or exposed by autoradiography. d HCT116 shUHRF1 cells overexpressing TIP60 and UHRF1 WT or UHRF1 4KR were immunoprecipitated using anti-pan-acetyl antibodies and immunoblotted with indicated antibodies.