Fig. 4.

TIP60-mediated UHRF1 acetylation is crucial for cell proliferation. a Cell viability was determined by MTT assay. HCT116 shUHRF1 cells were transfected with UHRF1 WT or UHRF1 4KR. Results were shown as mean ± SEM, n = 3; *p < 0.05. b Representative colony formation assay using HCT116 shUHRF1 cells expressing UHRF1 WT and UHRF1 4KR. Cells were incubated in fresh media for 7 days. Results were shown as mean ± SEM, n = 3; **p < 0.01. c RT-qPCR analysis of JDP2, FBLN2, UCHL1 gene using HCT116 control and shUHRF1 cells transfected with EV, UHRF1 WT and 4KR. Results were shown as mean ± SEM, n = 3; *p < 0.05, **p < 0.01, n.s: no significant difference. d HCT116 shUHRF1 cells transfected with UHRF1 WT and 4KR were fixed and stained with 7-Aminoactinomycin D (7-AAD), and the DNA content was measured by fluorescence-activated cell sorting (FACS) analysis. Data are presented as mean ± SEM, n = 3; **p < 0.01, n.s: no significant difference. e HCT116 shUHRF1 cells transfected with UHRF1 WT and 4KR were treated with 10 µM BrdU using pulse labelling for 30 min were fixed, immunostained with anti-BrdU-APC for 1 h and stained with 7-AAD for 5 m. BrdU-positive cells were measured by FACS analysis. Data are presented as mean ± SEM, n = 3; **p < 0.01.