Skip to main content
. 2022 Sep 29;23(19):11519. doi: 10.3390/ijms231911519

Figure 2.

Figure 2

VASP phosphorylation is enhanced, while Rap1b-GTP formation is reduced in platelets from PDS25 stimulated with 2-MeSADP. (A) Representative Western blot showing VASP phosphorylation in response to either 100 nM 2-MeSADP or 10 mM epinephrine for 3 min with or without PGE1 pretreatment. Total VASP was used to assess loading. (B) Quantitation of VASP phosphorylation as described in A. (C) Representative Western blot showing Rap1b-GTP in platelets from PDS25 or normal control donors stimulated with 100 nM 2-MeSADP alone or in combination with 10 μM epinephrine. Rap1b was used to assess loading. (D) Quantitation of GTP-Rap1b expressed as a ratio of total Rap1b as seen in C. H = Healthy control, P = PDS25. * p < 0.05 vs. control, n = 3.