Figure 3.
Gz-mediated aggregation is intact in platelets from PDS25, while thrombus formation on collagen is inhibited in whole blood from PDS25. (A) Representative aggregation tracings of platelets isolated from PDS25 or healthy controls stimulated with 100 nM 2-MeSADP, 10 μM epinephrine, or both. (B) Quantitation of aggregation as represented in panel A. * p < 0.05 vs. healthy control stimulated with 100 nM 2-MeSADP, n = 4. (C) Representative images of thrombus formation following whole blood (from PDS25 or healthy controls) flow over a collagen-coated surface. (D) Quantitation of thrombus area resulting from whole blood flow over collagen. * p < 0.05 vs. healthy control, n = 5.