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General principles in forming a minicircle from a parental plasmid. (A) The bacterial elements are removed from the parental plasmid by different enzymatic options (see main text). (B) The elements included in the minicircle can be optimised depending on the cell target, such as the photoreceptor cells. bGH = bovine growth hormone; S/MAR = scaffold matrix attachment region; WPRE = Woodchuck hepatitis virus post-transcriptional regulatory element.
