Table 1.
Summary of studies investigating plasmid and minicircle delivery to the eye.
| Species | DNA Structure | Other Element(s) | Delivery Mode(s) | Surgical Method | Transfected Cell Types | Reference |
|---|---|---|---|---|---|---|
| Rats (adult) | Minicircle and plasmid | Transfection reagent (Lipofectamine 2000) and Niosomes | Intravitreal injection | Ganglion cells | [24] | |
| Minicircle and plasmid | Transfection reagent (Lipofectamine 2000) and Niosomes | Subretinal injection | Inner nuclear layer and RPE | [24] | ||
| Mice (pups) | Plasmid | S/MAR | Transfection reagent (FuGENE) | Intravitreal injection | Ganglion cells | [30] |
| Mice (adult) | Plasmid | VMD2 promoter and S/MAR | Naked DNA and nanoparticle | Subretinal injection | RPE | [31] |
| Rpe65-/- mice (adult) | Plasmid | VMD2 promoter and S/MAR | Naked DNA and nanoparticle | Subretinal injection | RPE | [31,66] |
| Mice and rats (pups) | Plasmid | Electroporation | Subretinal injection | All retinal layers | [49] | |
| RHO P23H mice (pups) | Plasmid | Electroporation | Subretinal injection | Photoreceptors | [50] | |
| Mice (adult) | Plasmid | VMD2 promoter | Electroporation | Subretinal injection | RPE | [51] |
| Mice (adult) | Plasmid | VMD2 promoter | Electroporation | Subretinal injection | RPE | [52] |
| Rats (adult) | Plasmid | Electroporation | Intravitreal injection | Ganglion cells | [53] | |
| Plasmid | Electroporation | Subretinal injection | RPE | [53] | ||
| Mice (adult) | Plasmid | Iontophoresis | Photoreceptors | [55] | ||
| rd1 mice (pups) | Plasmid | PDE6B promoter | Iontophoresis | Photoreceptors | [55] | |
| Mice (adult) | Plasmid | Transfection reagent (Lipofectamine 2000 and NeuroPorter) | Subretinal injection | RPE | [56] | |
| Rats (adult) | Plasmid | Chloroquine-containing niosomes | Subretinal injection | Photoreceptor and RPE cells | [57] | |
| Mice (adult) | Plasmid | Transfection reagent (PEI) | Intravitreal injection | Ganglion cells | [59] | |
| Mice (adult) | Plasmid | CK30PEG nanoparticles | Subretinal injection | Photoreceptors | [61] | |
| rds mice (pups and adult) | Plasmid | RHO promoter | Naked DNA and CK30PEG nanoparticles | Subretinal injection | Photoreceptors | [64] |
| Abca4-/- mice (adult) | Plasmid | IRBP or MOP promoter | Naked DNA and CK30PEG nanoparticles | Subretinal injection | Photoreceptors | [65] |
| Rho-/- mice (pups) | Plasmid | MOP promoter and S/MAR | CK30PEG nanoparticles | Subretinal injection | Photoreceptors | [67] |