Figure 4.

Changes of weight (A), food intake (B), and water intake (C) in DIO rats treated with ASK2131 after 6 days of baseline measures, starting dose 1 nmol/kg day, then increase to 2, 5, 10, 20, 50, and finally 100 nmol/kg every 2 days. The low dose of ASK2131 was effective in significantly reducing body weight and food intake, higher doses did not result in greater reductions of food intake and body weight. Analysis by Two-Way ANOVA test with Šídák’s multiple comparisons test. * p < 0.05 vs. baseline, ** p < 0.01 vs. baseline, *** p < 0.0001 vs. baseline.