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. 2022 Oct 10;23(19):12030. doi: 10.3390/ijms231912030

Figure 1.

Figure 1

RNA sequencing of the ipsilateral and contralateral hippocampal dentate gyri during epileptogenesis. (A) Timeline and schematic of the experimental design. Mice were injected unilaterally in the hippocampus with kainate to induce SE or with saline control and sacrificed 3 days, 7 days, and 14 days after injection. The bilateral dentate gyri were harvested, and the dorsal portions underwent RNA sequencing. (B) Representative DAPI-stained brightfield coronal sections of anatomically dissected ipsilateral and contralateral dentate gyrus; scale bar 100 µm. Hierarchical cluster analysis demonstrating (C) the top 50 genes and (D) genes across the entire transcriptome with FDR < 0.01 and FC > ±1.5 dysregulated in the EZ and PIZ 3 days after SE induction across all samples. n = 4 mice per experimental group (2 males and 2 females).