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. 2022 Sep 21;23(19):11061. doi: 10.3390/ijms231911061

Figure 7.

Figure 7

Alcian blue 0.05% with MgCl2 0.05 M (A) and MgCl2 0.3 M (B) of human muscle samples, and biotinylated HABP immunohistochemistry staining of the human (C,D) and mouse (G–I) muscle samples. epi: epimysium, peri: perimysium, endo: endomysium. Scale bars: 50 μm. The Purple–Jelley HA assay showed the HA amount in human ((E): young vs. elderly women, * p = 0.04; (F): elderly men vs. elderly women), and in the mouse skeletal muscle ((J), * p < 0.05, ** p < 0.01). 6 W: 6 weeks old (Group A, puberty); 8 M: 8 months old (Group B, middle age); 2 Y: 2 years old (Group C, elderly).