Table 4.
The percentage area of collagen and the AOD (average optical density) of COLI and COLIII in the skeletal muscle of the mice.
| Characteristic | Group A (n = 5) |
Group B (n = 5) |
Group C (n = 5) |
A vs. B p-Value |
A vs. C p-Value |
B vs. C p-Value |
|---|---|---|---|---|---|---|
| # Collagen content (% area) | 2.05 ± 0.44 | 3.26 ± 0.86 | 7.97 ± 0.80 | 0.071 | <0.001 *** | <0.001 *** |
| # AOD of COLI | 0.17 ± 0.01 | 0.22 ± 0.02 | 0.30 ± 0.03 | 0.013 * | 0.002 ** | <0.001 *** |
| $ AOD of COLIII | 0.23 ± 0.08 | 0.23 ± 0.04 | 0.22 ± 0.05 | 0.994 | 0.897 | 0.982 |
ANOVA with Tukey post-hoc test #/Games–Howell post-hoc test $, AOD: average optical density, Group A = 6 weeks old (considered the pubertal group), Group B = 8 months old (considered the middle-aged group), Group C = 2 years old (considered the elderly group). The values are presented as numbers or means ± SD, at 40× magnification. * p < 0.05, ** p ≤ 0.01, *** p ≤ 0.001.