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. 2022 Sep 22;23(19):11148. doi: 10.3390/ijms231911148

Figure 2.

Figure 2

The expression of NF-κB p65 and A20 in senescent endothelial cells. The expression of p65, phosphorylated p65 (Pho-p65), and A20 (a negative regulator of NF-κB) was analyzed with senescent (S) and non-senescent (NS) human umbilical vein endothelial cells (HUVECs) by Western blotting. The relative expression of total p65:GAPDH (A), Pho-p65:GAPDH (B), Pho-p65:total p65 (C), and A20:GAPDH (D) of senescent cells was expressed as a ratio to non-senescent cells. Data are the mean ± standard deviation (SD) of six independent experiments. Values were compared between senescent and non-senescent cells. The expression of total p65 and Pho-p65 was upregulated in senescent cells. In contrast, the expression of A20 was downregulated in senescent cells. * p < 0.05, ** p < 0.01 [93].