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. 2022 Sep 22;23(19):11148. doi: 10.3390/ijms231911148

Figure 3.

Figure 3

A comparison of the levels of lipopolysaccharide (LPS)-induced ICAM-1 expression and NF-κB p65 phosphorylation in senescent and non-senescent endothelial cells. Senescent and non-senescent human umbilical vein endothelial cells (HUVECs) were incubated with or without LPS (10 or 100 ng/mL) for 24 h, and the expression of ICAM-1, p65, and phosphorylated p65 (Pho-p65) was analyzed by Western blotting. The relative expression of ICAM-1:GAPDH (A) and Pho-p65:total p65 (B) was expressed as a ratio to control cells (0) incubated without LPS in senescent (S) and non-senescent (NS) cells. Data are the mean ± standard deviation (SD) of four independent experiments. Values were compared with (100 ng/mL) and without (0 ng/mL) LPS incubation, as well as between senescent and non-senescent cells incubated with LPS (100 ng/mL). LPS-induced ICAM-1 expression and Pho-p65 level were enhanced in senescent cells. * p < 0.05, ** p < 0.01, and *** p < 0.001 [93].