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Primary and secondary antibodies.
| Antibody | Origin | Target | Concentration | Company |
|---|---|---|---|---|
| Primary | rabbit | claudin-1, -2, -3, -5, -7 | 1:1000 | Thermo Fisher Scientific, Waltham MA, USA |
| mouse | claudin-4 | 1:5000 | ||
| mouse | claudin-5 | 1:1000 | ||
| mouse | β-actin | 1:10,000 | Sigma-Aldrich Chemie, Steinheim, Germany | |
| Secondary | goat | rabbit/mouse IgG-F(ab′)2 fragment (HRP) | 1:10,000 | Jackson Immuno Res., West Grove PA, USA |
