Figure 5.

OsNAC15 functions as a transcription factor. (A) Transactivation activity of OsNAC15 in yeast. Schematic diagrams of the full-length and truncated OsNAC15 constructs are shown in the left panel. Yeast strain AH109 was used in the transactivation activity analysis. The transformants were streaked on the SD-Trp/-His media (right panel). (B) Yeast one-hybrid assay: OsNAC15 binds to the ZDRE motif; 100 mM 3-Amino-1,2,4-triazole (3-AT) was added in the SD-Leu/-Trp/-His medium. Interaction between OsbZIP46 and RAB21 was used as the positive control. Dilutions of 10×, 100× and 1000× correspond to an OD600 of 0.1, 0.01 and 0.001, respectively.