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. 2022 Oct 4;23(19):11771. doi: 10.3390/ijms231911771

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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OsNAC15 binds to the promoters of OsZIP7 and OsZIP10. (A,B) Yeast one-hybrid assay: 100 mM 3-AT was added in the SD-Leu/-Trp/-His medium. Interaction between OsbZIP46 and RAB21 was used as the positive control. Dilutions of 10×, 100× and 1000× correspond to an OD600 of 0.1, 0.01 and 0.001. (C) Transient expression assay in tobacco leaves. Firefly luciferase (LUC) activity was normalized to Renilla luciferase (REN) activity. Schematic representation of various constructs used in the assay is shown in the left panel. (D) Electrophoretic mobility shift assay. The competitor probe was added in 25-fold molar excess of labeled probes. Values are means ± SD of three biological replicates. *** p < 0.001 by Student’s t-test.