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. 2022 Oct 4;23(19):11782. doi: 10.3390/ijms231911782

Figure 1.

Figure 1

Experimental scheme. Spheroids were formed by “hanging drop” from day −3 to day 0 (d −3–d0) then transferred into low adhesion Petri dishes on d0. Spheroids were left to compact for 72 h (d0–d3) and from d3 to d10 EVsEXT were collected. On d10, spheroids were disaggregated as described in Methods and EVsINT were isolated. “Day 0” was chosen to signify the day spheroids were moved from drop to Petri; from day zero (d0) the previous 3 days (as d −1, d −2, d −3) and the following 10 (d1–d10) were considered.