Figure 1.

Conceptual scheme for conversion of PET to MA with an engineered multifunctional Pseudomonas putida. A leaf-branch compost cutinase (LCC) crude enzyme prepared from the culture supernatant hydrolyzes PET and produces the EG and TPA monomers, which can be directly used as substrates for the following cultivation to produce MA and reproduce LCC. The final product MA can be separated from the LCC crude enzyme by filtration. The remaining concentrated LCC crude enzyme was collected and used for the next round of PET hydrolysis with a stable activity, leading to the continuous conversion of PET to MA.