Table 1.
Strains and plasmids used in this study.
| Strains and Plasmids | Relevant Properties | Sources |
|---|---|---|
| pK18mobsacB | Allelic exchange vector, oriColE1 Mob+, sacB, Kmr | Lab Stock [39] |
| pBBR1MCS-2 | Protein expression vector, pBBR1 replicon, Mob+, Kmr | Lab Stock [40] |
| pBBR-LCC | LCC expression driven by lac promoter on pBBR1MCS-2 | This study |
| E. coli DH5α | F– φ80lacZ∆M15 ∆(lacZYA-argF)U169 deoR recA1 endA1 hsdR17(rK–, mK+) phoA supE44 λ– thi-1 gyrA96 relA1, used for plasmids construction and clone. | Lab Stock |
| P. putida KT2440 | Wild-type | Lab Stock |
| P. putida KT2440-t | P. putida KT2440 (ΔpcaHG::tph), genomic replacement of pcaHG with tph cluster (tphRA2A3A1BK). | This study |
| P. putida KT2440-ta | P. putida KT2440 (ΔpcaHG::tph::aroY:ecdB), additional insertion of codon-optimized aroY and ecdB follow tph cluster. | This study |
| P. putida KT2440-tac | P. putida KT2440 (ΔpcaHG::tph::aroY:ecdB ΔcatRBC::Ptac:catA), additional genomic replacement of catRBC with tac promoter, which enabled constitutive expression of catA | This study |
| P. putida KT2440-tacR | P. putida KT2440 (ΔpcaHG::tph::aroY:ecdB ΔcatRBC::Ptac:catA ΔgclR), additional gclR deletion. | This study |
| P. putida KT2440-tacRD | P. putida KT2440 (ΔpcaHG::tph::aroY:ecdB ΔcatRBC::Ptac:catA ΔgclR::Ptac:glcDEF), additional promoter replacement of glcDEF wih tac promoter. | This study |
| P. putida KT2440-tacRDL | P. putida KT2440 (ΔpcaHG::tph::aroY:ecdB ΔcatRBC::Ptac:catA ΔgclR::Ptac:glcDEF) (pBBR-LCC), additional LCC expression on pBBR1MCS-2 | This study |