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. 2022 Oct 2;23(19):11677. doi: 10.3390/ijms231911677

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Infiltrated immune cells in the tumor. Tumor tissue pre-treatment (obtained during the first craniotomy), post-first dose (obtained during the second craniotomy) and post-second dose (obtained during necropsy) (top to bottom in each row) were evaluated for the infiltration of B cells (CD20+), T cells (CD3+), regulatory T cells (FoxP3+), M1 macrophages (Mac387+) and activated microglia/macrophages (Iba1+). Scale bar: 50 μm. The graphic representation shows the area (%) of the positive cells (brown) for each marker.