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. 2022 Sep 28;23(19):11464. doi: 10.3390/ijms231911464

Figure 6.

Figure 6

DNA repair key players. (A) Relative gene expression of XRCC1/2/3, ERCC1, XPC, MSH2/6, PCNA, Rad51, and PARP-1 24 h after treatment with 4 Gy X-ray (light grey striped), proton (dark gray), and C-ions (dark grey dotted) IR in SW-1353 and Cal78 chondrosarcoma cells (mean ± SD; n = 4; measured in triplicates). Non-IR cells were used as controls, which is represented by the red dotted line (ratio = 1). Statistical significances are defined as follows: * p < 0.05; ** p < 0.01; *** p < 0.001. (B) Protein phosphorylation pattern of SW-1353 and Cal78 chondrosarcoma cells. The influence of IR on protein phosphorylation of p53, ATM, ATR, the TNF receptor TNFRST10B, and the DNA damage marker γH2AX was evaluated by immunoblotting under non-IR control conditions (ctrl) and 1 and 24 h after 2, 4, and 8 Gy X-ray/proton/C-ion IR. β-actin was used as loading control. Δ ratio, fold change normalized to non-IR controls (mean ± SD of n = 3). (C) The STRING database interaction network revealed the relationship of the most relevant cell cycle and DNA repair gene.