Figure 2.

H₂O₂ treatment and C2C12 myoblast viability. (A) C2C12 myoblasts were treated with different H₂O₂ concentrations (0.1–1 mM) for 24 h; (B) C2C12 myoblasts were treated with H₂O₂ 0.3 mM for different times (6–48 h). Cell viability was assessed by the trypan blue exclusion assay. Results are expressed as the number of cells (×1000). Data presented are the mean ± SEM of three experiments, each performed in triplicate. One-way ANOVA was performed followed by Bonferroni’s multiple comparisons test * p < 0.05; ** p < 0.01; **** p < 0.0001 vs. CTRL.