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. 2022 Sep 20;23(19):10986. doi: 10.3390/ijms231910986

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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H₂O₂ treatment and total antioxidant capacity. C2C12 myoblasts were treated with H₂O₂ 0.3 mM for different times (6–48 h) and cell lysates were tested for TAC analysis. TAC levels were expressed as micromoles of Trolox equivalents/mg of protein tested. Data presented are the mean ± SEM of three experiments, each performed in triplicate. One-way ANOVA was performed followed by Bonferroni’s multiple comparisons test **** p < 0.0001 vs. CTRL.