Figure 5.

Effect of Spd, AG, Spd-AG and H₂O₂ in single and combined treatments on C2C12 myoblast viability. (A) C2C12 myoblasts were treated with different Spd concentrations (1–100 μM) for 24 h; (B) C2C12 myoblasts were treated with H₂O₂ 0.3 mM, Spd 10 μM and AG 1mM in various combinations for 24 h. Cell viability was assessed by the trypan blue exclusion assay. Results are expressed as the number of cells (×1000). Data presented are the mean ± SEM of three experiments, each performed in triplicate. One-way ANOVA was performed, followed by Bonferroni’s multiple comparison tests for panels A and B, respectively. * p < 0.05; **** p < 0.0001.