Figure 7.
Glutathione homeostasis evaluation. (A) Total glutathione (tGSH); (B) oxidized glutathione (GSSG) and (C) reduced to oxidized glutathione ratio (GSH/GSSG) were evaluated in C2C12 myoblasts after 6 and 24 h in the presence of aminoguanidine (AG, 1 mM), spermidine (Spd, 10 μM), hydrogen peroxide (H2O2, 0.3 mM) and combined treatments. Data presented are the mean ± SEM of three experiments, each performed in triplicate. A two-way ANOVA test followed by Bonferroni’s multiple comparison test was performed. ** p < 0.01; *** p < 0.001; **** p < 0.0001.