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Effect of Spd-AG and H2O2 on C2C12 myoblasts on total antioxidant capacity. C2C12 myoblasts were treated with H2O2 0.3 mM, AG, 1 mM, Spd, 10 μM and combined treatments for 24 h. Cell lysates. TAC was expressed as micromole Trolox equivalents/mg of protein tested. Data presented are the mean ± SEM of three experiments, each performed in triplicate. A one-way ANOVA followed by Bonferroni’s multiple comparisons test was performed. * p < 0.05.
