Figure 10.

Effect of Spd-AG and H₂O₂ on the expression level of polyamines enzymatic pathway. C2C12 were treated with Spd 10μM in combination with AG 1 mM and/or H₂O₂ 0.3 mM for 6 h and RNA transcript expression of the polyamine enzymatic pathway was evaluated. qRT-PCR of biosynthetic enzymes (A) ornithine decarboxylase (ODC), spermidine synthase (SDS), spermine synthase (SMS), or catabolic enzymes (B) spermine oxidase (SMOX), spermine-/spermidine-N1-acetyltransferase 1 (SAT1) and polyamine oxidase (PAOX) amplified from C2C12 myoblasts were assayed. Data are calculated relative to the internal housekeeping gene (GAPDH) and are expressed as the mean fold change compared with control. Each value represents the mean ± SEM of three independent experiments, each performed in triplicate. One-way ANOVA followed by Bonferroni’s multiple comparisons test was performed * p < 0.05; ** p < 0.01.