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. 2022 Sep 21;23(19):11057. doi: 10.3390/ijms231911057

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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(A) Comparison of fold changes (FCs) in mRNA expression levels between P0 and E20 as obtained by hybridization to Affymetrix GeneChip versus RTqPCR for the number of selected up- and downregulated genes. There are no error bars, since data are presented as fold changes. Note: Pnlip: pancreatic lipase; Bcat2: branched-chain amino acid transaminase 2; Gas6: growth-arrest-specific 6; Afp: alpha-fetoprotein; Ngn3: neurog3; Srebf1/2: sterol regulatory element binding protein 1/2; Ins2: insulin 2; Pdx1: pancreatic duodenal homeobox 1; Egr1: early growth response factor 1. (B) Dendrogram for clustering using centered correlation and average linkage. Samples from P0 and P2 cluster closer together, indicating a more similar profile compared to samples from E20. (C) Heat map generated from the dCHIP hierarchical cluster analysis. Note: E20: embryonic day 20; P0: day 0 postpartum; P2: day two postpartum. Blue: Low expression. Red: High expression.