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. 2022 Sep 21;23(19):11057. doi: 10.3390/ijms231911057

Figure 2.

Figure 2

Expression and cellular localization of Afp in rat pancreas samples at E20, P0, and P2. (A,C,E) In situ hybridization of Afp mRNA using a dioxigenin-labeled LNA™ probe targeted against the same rat Afp sequence as the primers for RTqPCR (Qiagen, Exiqon, Vedbaek, Denmark) at E20 (A), P0 (C), and P2 (E). Afp is primarily expressed in association with islets of Langerhans in vessels and intra-islet capillaries. (B,D,F) Immunohistochemical detection (200× magnification) of Afp protein in 3 µm tissue sections from rat pancreas samples at E20 (B), P0 (D), and P2 (F). Afp is primarily detected in association with the endocrine compartments or ducts (arrows). Inserts at 400× magnification. (G) RTqPCR of Afp mRNA in rat pancreas samples at E20, P0, and P2. The mRNA values are normalized to Rpl13alpha. Afp mRNA expression is significantly increased at P0 compared to E20 and P2 (two-tailed t-test, p < 0.01). Note: *** p < 0.001.