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. 2022 Sep 21;23(19):11057. doi: 10.3390/ijms231911057

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Relative Ins2, Pdx-1, Neurog3, Ghr, and Prlr mRNA expression in perinatal rat pancreas from control (Ctrl) and low-protein (LP) diet animals. Messenger RNA levels measured using RTqPCR in whole rat pancreas samples at days E20, P0, and P2. Values are normalized to levels at E20 within each data set, n = 3–6. Data are given as means + SEM. (A) Ins2, (B) Pdx1, (C) Neurog3, (D) Ghr, (E) Prlr, and (F) immunohistochemical stainings for Neurog3 in the perinatal rat pancreas at P0 (200× magnification). Data in panels (A–E) were tested using 2-way ANOVA with Bonferroni post tests for control vs. LP animals. Asterisks indicate significance levels of individual comparisons. Note: ** p < 0.01, *** p < 0.001.