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. 2022 Sep 23;23(19):11224. doi: 10.3390/ijms231911224

Figure 2.

Figure 2

Acute METH exposure increases permeability within the colon. Colon cross-sections of sham-treated (A,A’,A’’,A’’’) and METH-treated (B,B’,B’’,B’’’) mice. (A,B) DAPI nuclei staining, (A’,B’) tight junction proteins labeled with anti- zonula occludents-1 (ZO-1) antibody, (A”,B’’) epithelial cells labeled with anti-epithelial cell adhesion molecule (EpCAm) antibody, and (A’’’,B’’’) merged images of DAPI, ZO-1, and EpCAm. (C,C’) Secondary Antibody Control. To show the non-specific binding of the secondary antibody, the primary antibody was omitted. Colon cross-sections; 20 μm. Images captured at 40×, scale bar: 50 μm. (D) Significant differences between sham-treated and METH-treated mice in tight junction permeability, epithelial cells, and concentration of fatty acid-binding protein (FABP)-1 in serum are shown. Results are expressed as mean ± standard error of the mean (SEM), * p < 0.05, ** p < 0.01.