Figure 3.

Acute METH exposure induces an inflammatory response within the colon. Colon cross-sections of sham-treated (A,A’,A’’) and METH-treated (B,B’,B’’) mice. (A,B) DAPI nuclei, (A’,B’) immune cells labeled with pan-leukocyte anti-CD45 antibody, (A’’,B’’) merged images of DAPI and CD45. (C) The primary antibody was omitted to show the non-specific binding of the secondary antibody. Colon cross-sections; 20 μm. Images captured at 40×, scale bar: 50 μm. (D) A significant difference between sham- and METH-treated mice in CD45 expression is noted. Results are expressed as mean ± standard error of the mean (SEM), * p < 0.05.