Table 4.
Plasmid information of Cas13 system used in the previous studies.
| Cas Effectors | Name of Plasmid Vector | Use | References |
|---|---|---|---|
| Cas13a | pET-Sumo-LbuCas13a | Expression plasmid | [73] |
| pCas13a-gRNA | Antiviral strategy | [64] | |
| pET-Lsh.Cas13a vector | Disease treatment | [74] | |
| pC013-Twinstrep SUMO-huLwCas13a | Detection and diagnosis | [75] | |
| pMD19T-E | Detection and diagnosis | [76] | |
| pC016-LwCas13a-GFP | Disease treatment | [77] | |
| pC016-LwCas13a-Ctrl | Disease treatment | [77] | |
| pC016-LwCas13a-RdRP | Disease treatment | [77] | |
| pC016-LwCas13a-PPIB | Disease treatment | [77] | |
| pC016-LwCas13a-CXCR4 | Disease treatment | [77] | |
| pC016-LwCas13a-KRAS | Disease treatment | [77] | |
| pC016-LwCas13a-N | Disease treatment | [77] | |
| pET28a-Cas13a-XLCHN-DTR-His | Disease treatment | [77] | |
| pK2GW7-pCas13a vector | Plant resistance | [78] | |
| pC016 LwCas13a | Cancer treatment | [79] | |
| pC034-LwCas13a-msfGFP-2A-Blast | Cancer treatment | [79] | |
| Lentiviral vector (unnamed) | Cancer treatment | [80] | |
| pET-Sumo-LbuCas13a expression vector | miRNA detection | [81] | |
| pDUAL-HFF1-Cas13a expression vectors | Retrovirus interference | [82] | |
| pKS-rrk1-(LshCas13a crRNA)-Control | Retrovirus interference | [82] | |
| pET-Sumo-LbuCas13a expression vectors | Basic research | [83] | |
| pUb LwaCas13a + LwaCas13a Guide RNA | Expresses LwaCas13a and guide RNA | [84] | |
| pLsCas13aGG | Backbone plasmid | [85] | |
| puc19-pCas13a | Intermediate/cloning vector | [86] | |
| pC015-dLwCas13a-NF | Expresses negative | [46] | |
| pC014-LwCas13a-msfGFP | Expresses active LwCas13a | [46] | |
| pC035-dLwCas13a-msfGFP | Expresses catalytically inactive LwCas13a | [46] | |
| pGJK_His-SUMO-LbuCas13a | Bacterial expression | [87] | |
| pDuBir-Lbu-dCas13a-avitag | Dual expression of Lbu-dCas13a and BirA | [88] | |
| pC0056-LwCas13a-msfGFP-NES | Expresses active LwaCas13a-NES | [46] | |
| pC034-LwCas13a-msfGFP-2A-Blast | Expresses active LwCas13a | [46] | |
| p2CT-His-MBP-Lwa_Cas13a_WT | Bacterial expression for Cas13a | [42] | |
| p2CT-His-MBP-Lne_Cas13a_WT | Bacterial expression for Cas13a | [42] | |
| p2CT-His-MBP-Lba_Cas13a_WT | Bacterial expression for Cas13a | [42] | |
| p2CT-His-MBP-Ere_Cas13a_WT | Bacterial expression for Cas13a | [42] | |
| p2CT-His-MBP-Cam_Cas13a_WT | Bacterial expression for Cas13a | [42] | |
| p2CT-His-MBP-Rca_Cas13a_WT | Bacterial expression for Cas13a | [42] | |
| p2CT-His-MBP-Hhe_Cas13a_WT | Bacterial expression for Cas13a | [42] | |
| p2CT-His-MBP-Ppr_Cas13a_WT | Bacterial expression for Cas13a | [42] | |
| pHAGE-IRES-puro-NLS-dLwaCas13a-EGFP-NLS-3xFlag | Overexpression | [58] | |
| pHAGE-IRES-puro-NLS-dLbaCas13a-EGFP-NLS-3xFlag | Overexpression | [58] | |
| Cas13b | pUb PspCas13b + PspCas13b guide RNA | Expresses PspCas13b | [84] |
| pBzCas13b/pPbcas13b/pBzCas13b/pBzCas13b-HEPN | Bacterial expression for Cas13b | [43] | |
| pC0041-RanCas13b crRNA backbone | For cloning of guide RNAs compatible with RanCas13b | [89] | |
| pU6-PspCas13b-gRNA-Actb1216 | PspCas13b guide RNA | [90] | |
| pAB1620 hU6-BpiI-Cas13bt3-DR | hU6-BpiI-Cas13bt3-DR (crRNA expression) | [38] | |
| Cas13d | pUb RxCas13d + RxCas13d guide RNA | Expresses RxCas13d | [84] |
| pUb dRxCas13d + RxCas13d guide RNA | Expresses catalytic dead RxCas13d | [84] | |
| pT3TS-RfxCas13d-HA | Plasmid to carry out IVT of RfxCas13d | [91] | |
| pET28a-MH6-EsCas13d | Expresses E. coli codon-optimized EsCas13d | [36] | |
| pET28a-MH6-RspCas13d_RspCasWYL1 | Expresses E. coli codon-optimized RspCas13d and RspCasWYL1 | [36] | |
| pT3TS-RfxCas13d-NLS-HA | Plasmid to carry out IVT of RfxCas13d-NLS | [91] | |
| dCas13d-dsRBD-APEX2 | TetON-APEX2-V5-BPNLS-dRfxCas13d-dsRBD-BPNLS-P2A-GFP | [92] | |
| pET-28b-RfxCas13d-His | Plasmid for bacterial expression and purification of RfxCas13d protein | [91] | |
| pSLQ5428_pHR_EF1a-mCherry-P2A-Rfx_Cas13d-2xNLS-3xFLAG | Lentiviral vector encoding Rfx Cas13d fused with 2xNLS, 3xFLAG, and 2A-tagged mCherry | [61] | |
| pLentiRNAGuide_002-hU6-RfxCas13d-DR-BsmBI-EFS-Puro-WPRE | For cloning of guide RNAs libraries compatible with RfxCas13d | [93] | |
| Cas13X | CMV-Cas13X.1-SV40pA_U6-BbsI-DR_CMV-mCherry-BGHpA | Expression vector for encoding a human codon-optimized Cas13X.1 driven by CMV promoter | [13] |
| CMV-dCas13X.1-REPAIRv2-SV40pA_CMV-mCherry-BGHpA_U6-BbsI-DR | Expression vector for encoding a human codon-optimized dCas13X.1-REPAIRv2 driven by CMV promoter | [13] | |
| U6-BbsI-DR_CMV-minidCas13X.1-REPAIRv2-BGHpA_CMV-EGFP-BGHpA | Expression vector for encoding a human codon-optimized minidCas13X.1-REPAIRv2 driven by CMV promoter | [13] | |
| CMV-dCas13X.1-RESCUE-S-SV40pA_U6-BbsI-DR_CMV-mCherry-P2A-Puro-BGHpA | Expression vector for encoding a human codon-optimized dCas13X.1-RESCUE-S driven by CMV promoter | [13] | |
| CMV-minidCas13X.1-RESCUE-S-SV40pA_U6-BbsI-DR_CMV-mCherry-P2A-Puro-BGHpA | Expression vector for encoding a human codon-optimized minidCas13X.1-RESCUE-S driven by CMV promoter | [13] |