Figure 6.

DIM treatment enhances antioxidant ability by activating Nrf2/HO-1 signaling pathway and reducing oxidative stress in TGF-β1-induced hepatocytes injury. (A) Protein expression levels of Nrf2, NQO1, Keap-1, HO-1, Cu/Zn SOD, and hepatic cytochrome P450 (CYP2E1) at 24 h after TGF-β1 treatment were determined by Western blot analysis. (B) Quantification of proteins expression normalized to β-actin. Data are expressed as mean ± SE of three individual experiments (n = 3). ^# p < 0.05, and ^### p < 0.001 denotes significant differences compared to the control; * p < 0.05, ** p < 0.01 and *** p < 0.001 compared to treatment with TGF-β1 only.