Figure 7.

DIM ameliorates CCl₄-induced mouse liver fibrosis. (A) Histopathological damage and fibrosis levels examined by H&E, Sirius Red, and Masson staining of liver; blue arrow indicates the levels of injury after CCl₄ treatment, and green arrow indicates the recovery after DIM treatment. Observed at 100× magnification. Scale bar: 100 µm. (B) Quantitative measurement (%) scoring of liver injury. (C,D) Statistics (%) of Sirius and Masson positive areas. Serum levels of alanine aminotransferase (ALT, (E)) and aspartate aminotransferase (AST, (F)) All data are presents as mean ± SD (n = 7). ^## p < 0.01, and ^### p < 0.001 denotes significant differences compared to the group treated with DIM only. * p < 0.05, ** p < 0.01, and *** p < 0.001 denote significant difference compared to the group treated with CCl₄.