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Effect of Ins and Rn on astrocytes in primary culture. Cell viability was determined by MTT assay in cells treated for 24 h. Astrocytes were incubated without Ins or Rn (control, C), with Rn (10−6 M), with Ins (10−8 M), or with Ins + Rn (10−6 M + 10−8 M). Data are mean ± SD of four independent experiments (four different rats). * p < 0.05 vs. control. + p < 0.05 vs. Rn. # p < 0.05 vs. Ins.
