Figure 2.

Effect of Ins and Ins + Rn on astrocytes in primary culture. Cells were isolated and seeded at 7 × 10⁴ cells/35 mm dish for 5 days. Cells were incubated without Ins or Rn (control, C), with Ins (10⁻⁸ M), or with Ins + Rn (10⁻⁸ + 10⁻⁶ M) for 24 h. Fluorescence products used were: Mitotracker (250 nM) (in green) to stain mitochondria, Lysotracker (250 nM) (in red) to stain lysosomes, and Hoechst 33342 (2 μg mL⁻¹) (in blue) to stain nuclei. Contrast images are added. Bar represents 20 μm.