Figure 4.
Effect of Ins and Rn on p-eNOS and eNos protein expression. Astrocytes were incubated without Ins or Rn (control, C), with Rn (10−6 M), with Ins (10−8 M), or with Ins + Rn (10−8 M + 10−6 M) for 24 h and collected to determine p-eNOS (1:250), eNOS (1:250), and Tubulin (1:3000) protein expressions by Western blot. A representative immunoblot is shown in the panel. Data are mean ± SD of four independent experiments (four different rats). * p < 0.05 vs. control. + p < 0.05 vs. Rn. # p < 0.05 vs. Ins.