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Effect of Ins and Rn on COX-2 protein expression. Astrocytes were incubated without Ins or Rn (control, C), with Rn (10−6 M), with Ins (10−8 M), or with Ins + Rn (10−8 M + 10−6 M) for 24 h and collected to determine COX-2 (1:500) and Tubulin (1:3000) protein expression by Western blot. A representative immunoblot is shown in the panel. Data are mean ± SD of four independent experiments (four different rats). * p < 0.05 vs. control. + p < 0.05 vs. Rn. # p < 0.05 vs. Ins.
