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. 2022 Oct 9;23(19):11969. doi: 10.3390/ijms231911969

Figure 7.

Figure 7

Effect of Ins and Rn on Cu/Zn-SOD (A) and Mn-SOD (B) protein expression. Astrocytes were incubated without Ins or Rn (control, C), with Rn (10−6 M), with Ins (10−8 M), or with Ins + Rn (10−8 M + 10−6 M) for 24 h and collected to determine Cu/Zn-SOD (1:500) (A) and Mn-SOD (1:250) (B), protein expression by Western blot. Tubulin (1:3000) was added in both figures as loading control. Representative immunoblots are shown in the panels. Data are mean ± SD of six independent experiments (six different rats). * p < 0.05 vs. control. + p < 0.05 vs. Rn. # p < 0.05 vs. Ins.