Figure 8.
Effect of Ins and Rn on NFκB (A) and IκB (B) protein expression. Astrocytes were incubated without Ins or Rn (control, C), with Rn (10−6 M), with Ins (10−8 M), or with Ins + Rn (10−8 M + 10−6 M) for 24 h and collected to determine NFκB (1:250) (A) and IκB (1:250) (B) protein expression by Western-blot. Tubulin (1:3000) was added in both figures as loading control). Representative immunoblots are shown in the panels. Data are mean ± SD of four independent experiments (four different rats).