Table 1.

Effect of Ins and Rn on astrocytes’ proliferation. Cell proliferation and counting living cells. Astrocytes were isolated and seeded at 7 × 10⁴ cells/35 mm dish for 5 days. Cells were incubated without Ins or Rn (control, C), with Rn (10⁻⁶ M), with Ins (10⁻⁸ M), or with Ins + Rn (10⁻⁸ + 10⁻⁶ M) for 24 h. Trypan blue exclusion was used to count the living cells and monitor cell proliferation. Data are mean ± SD of four independent experiments (four different rats). * p < 0.05 vs. control. # p < 0.05 vs. Ins.

	Seeding Cells (×104/35 mm Dish)	5 Days of Culture	24 h Treatment	% Proliferation
Control	7	12.86 ± 0.32	12.97 ± 0.24	0.85
Rn	7	12.87 ± 0.25	16.77 ± 0.35	30.31 *
Ins	7	12.85 ± 0.23	16.60 ± 0.37	29.18 *
Ins+Rn	7	12.88 ± 0.26	17.25 ± 0.35	33.91 *,#