Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Oct 1;23(19):11613. doi: 10.3390/ijms231911613

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

TRIM26 can ubiquitylate NEIL1, NTH1, OGG1, and NEIL3 in vitro. In vitro ubiquitylation of (A) His-tagged NEIL1 (4.6 pmol), (B) His-tagged NTH1 (5.8 pmol), (C) His-tagged OGG1 (5.2 pmol), and (D) His-tagged NEIL3 (3 pmol) by His-tagged TRIM26 (11 or 22 pmol). All in vitro ubiquitylation reactions were performed in the presence of E1 activating enzyme (0.7 pmol), H5a, H5b, and H5c E2-conjugating enzymes (2.5 pmol), and ubiquitin (0.6 nmol), and were subsequently analyzed by SDS-PAGE and immunoblotting using the respective antibodies. Molecular-weight markers are indicated on the left-hand side of the blots, and the positions of unmodified and ubiquitylated proteins (e.g., NEIL1_ub) are displayed.