Figure 2.

NEIL1 and OGG1 control cell survival in response to X-ray radiation in a TRIM26-dependent manner. (A,B,D,F,H) WCE from U2OS cells treated with NT control siRNA, plus the various combinations of trim26 knockdown alone and with DNA glycosylase (neil1, nth1, ogg1, or neil3) were prepared and analyzed by SDS-PAGE and immunoblotting using the indicated antibodies. (C,E,G,I) Clonogenic survival of cells was analyzed following treatment with increasing doses of X-ray irradiation (0–4 Gy). Shown is the mean surviving fraction ± S.E. from at least three independent experiments.