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. 2022 Oct 1;23(19):11613. doi: 10.3390/ijms231911613

Figure 3.

Figure 3

NEIL1 and OGG1 control the repair of DNA damage in response to X-ray radiation in a TRIM26-dependent manner. (A–C) U2OS cells treated with NT control siRNA, plus the combinations of trim26 knockdown alone and with either (A) neil1, (B) nth1, or (C) ogg1 siRNA were irradiated with X-ray irradiation (1.5 Gy) and alkali-labile sites and DNA single-strand breaks measured at 0–60 min post-treatment using the alkaline comet assay. Shown is the mean % tail DNA ± S.D. * p < 0.05, ** p < 0.02, and *** p < 0.01 as analyzed by a two-sample t-test. (D,G) WCE from U2OS cells with either (D) Flag-NEIL1 or (G) Flag-OGG1 overexpression were prepared and analyzed by SDS-PAGE and immunoblotting with the indicated antibodies. (E,F) Clonogenic survival of cells containing either (E) NEIL1 or (F) OGG1 overexpression was analyzed following treatment with increasing doses of X-ray irradiation (0–4 Gy). Shown is the mean surviving fraction ± S.E from at least three independent experiments. (H,I) U2OS cells containing either (H) NEIL1 or (I) OGG1 overexpression were irradiated with X-ray irradiation (1.5 Gy) and alkali-labile sites and DNA single-strand breaks measured at 0–60 min post-treatment using the alkaline comet assay. Shown is the mean % tail DNA ± S.D. * p < 0.05, ** p < 0.02, and *** p < 0.005 as analyzed by a two-sample t-test.